TY - JOUR
T1 - A neurotoxic phospholipase A2 variant: Isolation and characterization from eastern regional Indian cobra (Naja naja) venom
AU - Shashidharamurthy, Rangaiah
AU - Kemparaju, K.
AU - Taval, Shashidharamurthy
PY - 2006/1/1
Y1 - 2006/1/1
N2 - CM-Sephadex C-25 column chromatography profile of Indian cobra (Naja naja) venom from eastern region showed a distinct and a dominant phospholipase peak, peak-10, while it was not seen in either southern or western venom samples. Peak-10 was subjected to CM-Sephadex C-25 and Sephadex G-50 column chromatography to isolate NN-X-PLA2. NN-X-PLA2 is a single chain protein with the relative molecular weight of 10 kDa by SDS-PAGE. It was toxic to mice with an LD50 value 0.098 mg/kg body weight (i.p.) and the mice exhibited acute neurotoxic symptoms. Upon indirect stimulation, it inhibited the twitching of frog's gastrocnemius muscle in a dose dependent manner. NN-X-PLA2 was weakly anticoagulant and devoid of cytotoxicity, myotoxicity, hemorrhage, edema inducing, and directlytic activities and effects on platelet aggregation process. Upon chemical modification independently with p-bromophenacyl bromide and acetic anhydride, NN-X-PLA2 lost both enzymatic and toxic properties. © 2006 Elsevier Ltd. All rights reserved.
AB - CM-Sephadex C-25 column chromatography profile of Indian cobra (Naja naja) venom from eastern region showed a distinct and a dominant phospholipase peak, peak-10, while it was not seen in either southern or western venom samples. Peak-10 was subjected to CM-Sephadex C-25 and Sephadex G-50 column chromatography to isolate NN-X-PLA2. NN-X-PLA2 is a single chain protein with the relative molecular weight of 10 kDa by SDS-PAGE. It was toxic to mice with an LD50 value 0.098 mg/kg body weight (i.p.) and the mice exhibited acute neurotoxic symptoms. Upon indirect stimulation, it inhibited the twitching of frog's gastrocnemius muscle in a dose dependent manner. NN-X-PLA2 was weakly anticoagulant and devoid of cytotoxicity, myotoxicity, hemorrhage, edema inducing, and directlytic activities and effects on platelet aggregation process. Upon chemical modification independently with p-bromophenacyl bromide and acetic anhydride, NN-X-PLA2 lost both enzymatic and toxic properties. © 2006 Elsevier Ltd. All rights reserved.
KW - Naja naja venom
KW - Neurotoxic PLA2
KW - PLA2 variant
KW - Venom variability
UR - https://digitalcommons.pcom.edu/scholarly_papers/551
M3 - Article
VL - 47
JO - Toxicon
JF - Toxicon
ER -