Abstract
Long-term potentiation (LTP) is an activity-dependent enhancement of synaptic efficacy, considered a model of learning and memory. The biochemical cascade producing LTP requires activation of Src, which upregulates the function of NMDA receptors (NMDARs), but how Src becomes activated is unknown. Here, we show that the focal adhesion kinase CAKß/Pyk2 upregulated NMDAR function by activating Src in CA1 hippocampal neurons. Induction of LTP was prevented by blocking CAKß/Pyk2, and administering CAKß/Pyk2 intracellularly mimicked and occluded LTP. Tyrosine phosphorylation of CAKß/Pyk2 and its association with Src was increased by stimulation that produced LTP. Finally, CAKß/Pyk2-stimulated enhancement of synaptic AMPA responses was prevented by blocking NMDARS, chelating intracellular Ca2+, or blocking Src. Thus, activating CAKß/Pyk2 is required for inducing LTP and may depend upon downstream activation of Src to upregulate NMDA receptors.
| Original language | American English |
|---|---|
| Journal | Neuron |
| Volume | 29 |
| State | Published - Jan 1 2001 |
Keywords
- alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid
- cakbeta kinase
- calcium ion
- focal adhesion kinase
- n methyl dextro aspartic acid receptor
- protein tyrosine kinase
- pyk2 kinase
- unclassified drug
- AMPA receptor
- focal adhesion kinase 2
- Ptk2b protein
- rat
- animal cell
- article
- controlled study
- enzyme activity
- hippocampus
- long term potentiation
- nonhuman
- priority journal
- protein phosphorylation
- protein protein interaction
- signal transduction
- animal
- male
- metabolism
- physiology
- pyramidal nerve cell
- Sprague Dawley rat
- upregulation
- Animals
- Long-Term Potentiation
- Protein-Tyrosine Kinases
- Pyramidal Cells
- Rats
- Sprague-Dawley
- Receptors
- AMPA
- N-Methyl-D-Aspartate
- src-Family Kinases
- Up-Regulation
Disciplines
- Neuroscience and Neurobiology