Current Protocols in Neuroscience: Fluorescence Recovery after Photobleaching (FRAP) with a Focus on F-Actin

Lori R. Hardy, Lori Redmond

Research output: Book/ReportBook


In this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein-protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area. The data gained in FRAP studies is qualitative and yields insight into relative binding affinity, binding characteristics, and the effect of treatments or mutations on protein mobility.

Original languageAmerican English
PublisherCurrent Protocols in Neuroscience
VolumeChapter 2
StatePublished - Jan 1 2012


  • FRAP
  • actin
  • protein mobility
  • GFPactin cytoskeleton
  • actins
  • cells
  • cultured
  • fluorescence recovery after photobleaching
  • green fluorescent proteins
  • microscopy
  • confocal
  • time factors


  • Medicine and Health Sciences
  • Neurology
  • Neurosciences

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