Ginkgo Biloba aqueous extract attenuated MDMA-induced Neurodegeneration and its accompanying memory aberrations in experimental Wistar rats model

Olanrewaju John Afees; Joshua Owolabi; Simon Peniel Oluwatoni; P;atunji Sunday Yinka; Enya Joseph Igbo; Arietarhire Leviticus; Adelodun Stephen Taiye; Taiwo-ola Dorcas; Afolabi Toluwanimi; Fabiyi Oluwaseyi Sunday

doi:10.1016/j.phyplu.2021.100123

Ginkgo Biloba aqueous extract attenuated MDMA-induced Neurodegeneration and its accompanying memory aberrations in experimental Wistar rats model

Olanrewaju John Afees, Joshua Owolabi, Simon Peniel Oluwatoni, P;atunji Sunday Yinka, Enya Joseph Igbo, Arietarhire Leviticus, Adelodun Stephen Taiye, Taiwo-ola Dorcas, Afolabi Toluwanimi, Fabiyi Oluwaseyi Sunday

Department of Bio-Medical Sciences (PCOM)

Research output: Contribution to journal › Article › peer-review

Abstract

 Background
 3,4-methylenedioxymethamphetamine (MDMA) is a       psychoactive drug     that teenagers and young adults habitually abuse for pleasurable sensations and can cause neurological disorders. Aim: This research focuses on investigating the attenuating potential of       Ginkgo biloba       (G. biloba)   aqueous extract, following MDMA-induced       neurodegeneration     and its accompanying memory aberrations in rat model.
 Methods
 A total of thirty-two (32) male Wistar rats weighing approximately 140–160 g were randomly divided into four groups, and housed in plastic cages at room temperature. During experimental treatment: CTR-group were given normal saline daily, MDMA-group were given 10mg/kg of MDMA intraperitoneally (ip),    G. biloba  -group received 200 mg/kg of    G. biloba   orally and MDMA+    G. biloba  -group were given 10 mg/kg of MDMA followed by 200 mg/kg of    G. biloba.   Substance administration took 22 days.
 Results
 10 mg/kg ip injection of MDMA obviously reduced body weight and significantly increased the escape time as well as wrong holes poking with depletion in open field test parameters scored.    G. biloba   was able to ameliorate this. Correspondingly, MDMA treatment reduced the expression of p53,       p21    ,       GSH     and GPx-1 gene. Disruption in neuronal arrangement, mild depolarization and       vacuolation     of pyramidial neurons were seen in the histo-architecture.       Glial fibrillary acidic protein     (GFAP) expression revealed increased astrocyte densities (astrogliosis) in the hippocampus and Ki-67 staining demonstrated the degree of       cell proliferation     which highly decreased following 10 mg/kg ip injection of MDMA.
 Conclusion
 However, 200 mg/kg of    G. biloba   aqueous extract was able to restore MDMA-induced hippocampal damage over a period of time, with a need for further investigation to ascertain which phytoconstituent of    G. biloba   is more effective in the treatment of psychstimulant induced brain damage

Original language	American English
Journal	Phytomedicine Plus
Volume	1
DOIs	https://doi.org/10.1016/j.phyplu.2021.100123
State	Published - 2021

Keywords

3.4-methylenedioxymehampetamine (MDMA)
G. biloba
Hippocampus
Neuroanatomy
Neurodegeneration
neurotoxicity

Disciplines

Medicine and Health Sciences

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Cite this

@article{8d4cf7e563e7493d86065f26df14f3a6,

title = "Ginkgo Biloba aqueous extract attenuated MDMA-induced Neurodegeneration and its accompanying memory aberrations in experimental Wistar rats model",

abstract = " Background 3,4-methylenedioxymethamphetamine (MDMA) is a psychoactive drug that teenagers and young adults habitually abuse for pleasurable sensations and can cause neurological disorders. Aim: This research focuses on investigating the attenuating potential of Ginkgo biloba (G. biloba) aqueous extract, following MDMA-induced neurodegeneration and its accompanying memory aberrations in rat model. Methods A total of thirty-two (32) male Wistar rats weighing approximately 140–160 g were randomly divided into four groups, and housed in plastic cages at room temperature. During experimental treatment: CTR-group were given normal saline daily, MDMA-group were given 10mg/kg of MDMA intraperitoneally (ip), G. biloba -group received 200 mg/kg of G. biloba orally and MDMA+ G. biloba -group were given 10 mg/kg of MDMA followed by 200 mg/kg of G. biloba. Substance administration took 22 days. Results 10 mg/kg ip injection of MDMA obviously reduced body weight and significantly increased the escape time as well as wrong holes poking with depletion in open field test parameters scored. G. biloba was able to ameliorate this. Correspondingly, MDMA treatment reduced the expression of p53, p21 , GSH and GPx-1 gene. Disruption in neuronal arrangement, mild depolarization and vacuolation of pyramidial neurons were seen in the histo-architecture. Glial fibrillary acidic protein (GFAP) expression revealed increased astrocyte densities (astrogliosis) in the hippocampus and Ki-67 staining demonstrated the degree of cell proliferation which highly decreased following 10 mg/kg ip injection of MDMA. Conclusion However, 200 mg/kg of G. biloba aqueous extract was able to restore MDMA-induced hippocampal damage over a period of time, with a need for further investigation to ascertain which phytoconstituent of G. biloba is more effective in the treatment of psychstimulant induced brain damage",

keywords = "3.4-methylenedioxymehampetamine (MDMA), G. biloba, Hippocampus, Neuroanatomy, Neurodegeneration, neurotoxicity",

author = "Afees, {Olanrewaju John} and Joshua Owolabi and Oluwatoni, {Simon Peniel} and Yinka, {P;atunji Sunday} and Igbo, {Enya Joseph} and Arietarhire Leviticus and Taiye, {Adelodun Stephen} and Taiwo-ola Dorcas and Afolabi Toluwanimi and Sunday, {Fabiyi Oluwaseyi}",

year = "2021",

doi = "10.1016/j.phyplu.2021.100123",

language = "American English",

volume = "1",

journal = "Phytomedicine Plus",

}

TY - JOUR

T1 - Ginkgo Biloba aqueous extract attenuated MDMA-induced Neurodegeneration and its accompanying memory aberrations in experimental Wistar rats model

AU - Afees, Olanrewaju John

AU - Owolabi, Joshua

AU - Oluwatoni, Simon Peniel

AU - Yinka, P;atunji Sunday

AU - Igbo, Enya Joseph

AU - Leviticus, Arietarhire

AU - Taiye, Adelodun Stephen

AU - Dorcas, Taiwo-ola

AU - Toluwanimi, Afolabi

AU - Sunday, Fabiyi Oluwaseyi

PY - 2021

Y1 - 2021

N2 - Background 3,4-methylenedioxymethamphetamine (MDMA) is a psychoactive drug that teenagers and young adults habitually abuse for pleasurable sensations and can cause neurological disorders. Aim: This research focuses on investigating the attenuating potential of Ginkgo biloba (G. biloba) aqueous extract, following MDMA-induced neurodegeneration and its accompanying memory aberrations in rat model. Methods A total of thirty-two (32) male Wistar rats weighing approximately 140–160 g were randomly divided into four groups, and housed in plastic cages at room temperature. During experimental treatment: CTR-group were given normal saline daily, MDMA-group were given 10mg/kg of MDMA intraperitoneally (ip), G. biloba -group received 200 mg/kg of G. biloba orally and MDMA+ G. biloba -group were given 10 mg/kg of MDMA followed by 200 mg/kg of G. biloba. Substance administration took 22 days. Results 10 mg/kg ip injection of MDMA obviously reduced body weight and significantly increased the escape time as well as wrong holes poking with depletion in open field test parameters scored. G. biloba was able to ameliorate this. Correspondingly, MDMA treatment reduced the expression of p53, p21 , GSH and GPx-1 gene. Disruption in neuronal arrangement, mild depolarization and vacuolation of pyramidial neurons were seen in the histo-architecture. Glial fibrillary acidic protein (GFAP) expression revealed increased astrocyte densities (astrogliosis) in the hippocampus and Ki-67 staining demonstrated the degree of cell proliferation which highly decreased following 10 mg/kg ip injection of MDMA. Conclusion However, 200 mg/kg of G. biloba aqueous extract was able to restore MDMA-induced hippocampal damage over a period of time, with a need for further investigation to ascertain which phytoconstituent of G. biloba is more effective in the treatment of psychstimulant induced brain damage

AB - Background 3,4-methylenedioxymethamphetamine (MDMA) is a psychoactive drug that teenagers and young adults habitually abuse for pleasurable sensations and can cause neurological disorders. Aim: This research focuses on investigating the attenuating potential of Ginkgo biloba (G. biloba) aqueous extract, following MDMA-induced neurodegeneration and its accompanying memory aberrations in rat model. Methods A total of thirty-two (32) male Wistar rats weighing approximately 140–160 g were randomly divided into four groups, and housed in plastic cages at room temperature. During experimental treatment: CTR-group were given normal saline daily, MDMA-group were given 10mg/kg of MDMA intraperitoneally (ip), G. biloba -group received 200 mg/kg of G. biloba orally and MDMA+ G. biloba -group were given 10 mg/kg of MDMA followed by 200 mg/kg of G. biloba. Substance administration took 22 days. Results 10 mg/kg ip injection of MDMA obviously reduced body weight and significantly increased the escape time as well as wrong holes poking with depletion in open field test parameters scored. G. biloba was able to ameliorate this. Correspondingly, MDMA treatment reduced the expression of p53, p21 , GSH and GPx-1 gene. Disruption in neuronal arrangement, mild depolarization and vacuolation of pyramidial neurons were seen in the histo-architecture. Glial fibrillary acidic protein (GFAP) expression revealed increased astrocyte densities (astrogliosis) in the hippocampus and Ki-67 staining demonstrated the degree of cell proliferation which highly decreased following 10 mg/kg ip injection of MDMA. Conclusion However, 200 mg/kg of G. biloba aqueous extract was able to restore MDMA-induced hippocampal damage over a period of time, with a need for further investigation to ascertain which phytoconstituent of G. biloba is more effective in the treatment of psychstimulant induced brain damage

KW - 3.4-methylenedioxymehampetamine (MDMA)

KW - G. biloba

KW - Hippocampus

KW - Neuroanatomy

KW - Neurodegeneration

KW - neurotoxicity

U2 - 10.1016/j.phyplu.2021.100123

DO - 10.1016/j.phyplu.2021.100123

M3 - Article

VL - 1

JO - Phytomedicine Plus

JF - Phytomedicine Plus

ER -