Identification of Protein-Bound Dinitrosyl Iron Complexes by Nuclear Resonance Vibrational Spectroscopy

Zachary J. Tonzetich; Hongxin Wang; Devrani Mitra; Christine E. Tinberg; Loi H. Do; Francis E. Jenney; Michael W. W. Adams; Stephen P. Cramer; Stephen J. Lippard

Identification of Protein-Bound Dinitrosyl Iron Complexes by Nuclear Resonance Vibrational Spectroscopy

Zachary J. Tonzetich, Hongxin Wang, Devrani Mitra, Christine E. Tinberg, Loi H. Do, Francis E. Jenney, Michael W. W. Adams, Stephen P. Cramer, Stephen J. Lippard

Department of Bio-Medical Sciences (PCOM)

Research output: Contribution to journal › Article › peer-review

Abstract

We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.

Original language	American English
Journal	Journal of the American Chemical Society
Volume	132
State	Published - May 26 2010

Keywords

Archaeal Proteins
Ferredoxins
Iron
Nitrogen Oxides
Proteins
Pyrococcus furiosus
Spectrum Analysis
Vibration

Disciplines

Medical Biochemistry
Medicine and Health Sciences

Cite this

@article{d48edaec7022408da0e020a7a05a584c,

title = "Identification of Protein-Bound Dinitrosyl Iron Complexes by Nuclear Resonance Vibrational Spectroscopy",

abstract = " We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.",

keywords = "Archaeal Proteins, Ferredoxins, Iron, Nitrogen Oxides, Proteins, Pyrococcus furiosus, Spectrum Analysis, Vibration",

author = "Tonzetich, {Zachary J.} and Hongxin Wang and Devrani Mitra and Tinberg, {Christine E.} and Do, {Loi H.} and Jenney, {Francis E.} and Adams, {Michael W. W.} and Cramer, {Stephen P.} and Lippard, {Stephen J.}",

year = "2010",

month = may,

day = "26",

language = "American English",

volume = "132",

journal = "Journal of the American Chemical Society",

}

TY - JOUR

T1 - Identification of Protein-Bound Dinitrosyl Iron Complexes by Nuclear Resonance Vibrational Spectroscopy

AU - Tonzetich, Zachary J.

AU - Wang, Hongxin

AU - Mitra, Devrani

AU - Tinberg, Christine E.

AU - Do, Loi H.

AU - Jenney, Francis E.

AU - Adams, Michael W. W.

AU - Cramer, Stephen P.

AU - Lippard, Stephen J.

PY - 2010/5/26

Y1 - 2010/5/26

N2 - We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.

AB - We have applied (57)Fe nuclear resonance vibrational spectroscopy (NRVS) to identify protein-bound dinitrosyl iron complexes. Intense NRVS peaks due to vibrations of the N-Fe-N unit can be observed between 500 and 700 cm(-1) and are diagnostic indicators of the type of iron dinitrosyl species present. NRVS spectra for four iron dinitrosyl model compounds are presented and used as benchmarks for the identification of species formed in the reaction of Pyrococcus furiosus ferredoxin D14C with nitric oxide.

KW - Archaeal Proteins

KW - Ferredoxins

KW - Iron

KW - Nitrogen Oxides

KW - Proteins

KW - Pyrococcus furiosus

KW - Spectrum Analysis

KW - Vibration

UR - https://digitalcommons.pcom.edu/scholarly_papers/67

M3 - Article

VL - 132

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

ER -

Identification of Protein-Bound Dinitrosyl Iron Complexes by Nuclear Resonance Vibrational Spectroscopy

Abstract

Keywords

Disciplines

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