TY - JOUR
T1 - Improved detection of fetal cells from maternal blood with polymerase chain reaction
AU - Adkison, Linda R.
AU - Andrews, Roberta H.
AU - Vowell, Nannette L.
AU - Koontz, William L.
PY - 1994
Y1 - 1994
N2 - OBJECTIVE: The objective was to test the reliability of new deoxyribonucleic acid primers that have previously been used very efficiently by this laboratory with amniolysate samples to amplify a 248 bp Y-specific, repeated sequence from maternal blood during pregnancy. STUDY DESIGN: Blood samples were obtained from 50 women during weeks 11 and 16 of pregnancy and were analyzed for the presence of the Y chromosome-specific sequences. RESULTS: Y-specific fragments were identified in 19 of 24 (79.2%) women after one complete amplification. A second amplification of these samples negative for Y-specific fragments revealed three additional samples positive for the Y chromosome-specific fragment. Only two male fetuses remained unidentified. Overall, 91.7% male fetuses and 96% of all fetuses (48/50) in these women were correctly identified. CONCLUSIONS: The primers described in this study provide an additional or alternative tool for the determination, by means of the polymerase chain reaction, of Y chromosome-bearing cells in maternal circulation. (AM J OBSTET GYNECOL 1994;170:952-5.) © 1994, Mosby, Inc.. All rights reserved.
AB - OBJECTIVE: The objective was to test the reliability of new deoxyribonucleic acid primers that have previously been used very efficiently by this laboratory with amniolysate samples to amplify a 248 bp Y-specific, repeated sequence from maternal blood during pregnancy. STUDY DESIGN: Blood samples were obtained from 50 women during weeks 11 and 16 of pregnancy and were analyzed for the presence of the Y chromosome-specific sequences. RESULTS: Y-specific fragments were identified in 19 of 24 (79.2%) women after one complete amplification. A second amplification of these samples negative for Y-specific fragments revealed three additional samples positive for the Y chromosome-specific fragment. Only two male fetuses remained unidentified. Overall, 91.7% male fetuses and 96% of all fetuses (48/50) in these women were correctly identified. CONCLUSIONS: The primers described in this study provide an additional or alternative tool for the determination, by means of the polymerase chain reaction, of Y chromosome-bearing cells in maternal circulation. (AM J OBSTET GYNECOL 1994;170:952-5.) © 1994, Mosby, Inc.. All rights reserved.
KW - Fetal sex determination
KW - peripheral maternal blood
KW - polymerase chain reaction
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U2 - 10.1016/S0002-9378(94)70312-4
DO - 10.1016/S0002-9378(94)70312-4
M3 - Article
C2 - 8141230
AN - SCOPUS:0028225503
SN - 0002-9378
VL - 170
SP - 952
EP - 955
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
IS - 3
ER -