TY - JOUR
T1 - Increased Myo/Nog Cell Presence and Phagocytic Activity in Retinal Degeneration: Insights from a Mouse Model
AU - Crowley, Diana
AU - Murad, Samantha
AU - Helm, Courtney
AU - Souza, Rachel
AU - Coughlan, Sarah
AU - Serpico, Scott
AU - Sugarman, Eric
AU - Marguiles, Kyle
AU - Heist, Brian
AU - Mitchell, Kathryn D.
AU - Sutera, Christopher K.
AU - Martin, Mark
AU - Font, Carlos
AU - Woodruff, Mary
AU - Tsai, E-Jine
AU - Brahmbhatt, Rushil
AU - Lecker, Paul
AU - Gorski, Grzegorz
AU - Benalcazar, John
AU - Young, Serena
AU - Martin, Abey
AU - Gugerty, Lindsay
AU - Gerhart, Jacquelyn
AU - George-Weinstein, Mindy
AU - Nuevo, Arturo Bravo
PY - 2025/1/1
Y1 - 2025/1/1
N2 - Myo/Nog cells play a pivotal role in ocular development and demonstrate a rapid response to stress and injury. This study investigates their behavior and distribution in a murine model of retinitis pigmentosa, specifically in C3H/HeJ mice, which exhibit photoreceptor degeneration due to a homozygous mutation in the Pde6brd1 gene. Retinal samples from C3H/HeJ and C57BL/6J mice were analyzed at postnatal weeks 2.5 to 6 using hematoxylin and eosin staining, immunofluorescence for brain-specific angiogenesis inhibitor 1 (BAI1) expressed in Myo/Nog cells, and TUNEL labeling for apoptotic cell detection. The results demonstrated a progressive thinning of the outer nuclear layer (ONL) in C3H mice, accompanied by a significant increase in Myo/Nog cell numbers. In normal retinas, Myo/Nog cells were primarily located in the inner nuclear and outer plexiform layers. However, in C3H/HeJ mice, they accumulated in the ONL near apoptotic photoreceptors and within the choroid. Notably, in these degenerative regions, Myo/Nog cells exhibited features of phagocytosis, suggesting a role in apoptotic cell clearance. Additionally, parallels between Myo/Nog cell responses in retinitis pigmentosa and models of oxygen-induced retinopathy, ocular hypertension, and light damage suggest that these cells may be leveraged for therapeutic purposes.
AB - Myo/Nog cells play a pivotal role in ocular development and demonstrate a rapid response to stress and injury. This study investigates their behavior and distribution in a murine model of retinitis pigmentosa, specifically in C3H/HeJ mice, which exhibit photoreceptor degeneration due to a homozygous mutation in the Pde6brd1 gene. Retinal samples from C3H/HeJ and C57BL/6J mice were analyzed at postnatal weeks 2.5 to 6 using hematoxylin and eosin staining, immunofluorescence for brain-specific angiogenesis inhibitor 1 (BAI1) expressed in Myo/Nog cells, and TUNEL labeling for apoptotic cell detection. The results demonstrated a progressive thinning of the outer nuclear layer (ONL) in C3H mice, accompanied by a significant increase in Myo/Nog cell numbers. In normal retinas, Myo/Nog cells were primarily located in the inner nuclear and outer plexiform layers. However, in C3H/HeJ mice, they accumulated in the ONL near apoptotic photoreceptors and within the choroid. Notably, in these degenerative regions, Myo/Nog cells exhibited features of phagocytosis, suggesting a role in apoptotic cell clearance. Additionally, parallels between Myo/Nog cell responses in retinitis pigmentosa and models of oxygen-induced retinopathy, ocular hypertension, and light damage suggest that these cells may be leveraged for therapeutic purposes.
U2 - 10.3390/app15105486
DO - 10.3390/app15105486
M3 - Article
JO - Applied Sciences (Switzerland)
JF - Applied Sciences (Switzerland)
ER -