Abstract
Rheumatoid arthritis and periodontitis are chronic inflammatory diseases associated with tissue destruction that is mediated in part by elevated levels of cytokines (e.g. interleukin-1 and tumor necrosis factor). Differential screening of a human synovial fibroblast cDNA library for interleukin-1 induced genes revealed a clone identical to the gene encoding human bone morphogenetic protein-2. Northern blot analysis of human synovial fibroblast mRNA confirmed up-regulation of bone morphogenetic protein-2 in the presence of interleukin-1. Utilizing a specific antibody, levels of bone morphogenetic protein-2 protein in conditioned medium from synovial fibroblasts were also up-regulated in the presence of interleukin-1. This is the first report of the production of bone morphogenetic protein-2 by synovial fibroblasts, and the first report of its up-regulation in response to interleukin-1. However, interleukin-1 did not induce bone morphogenetic protein-2 mRNA in human gingival fibroblasts.
| Original language | American English |
|---|---|
| Journal | Biochemical and biophysical research communications |
| Volume | 248 |
| State | Published - Jan 1 1998 |
Keywords
- Bone Morphogenetic Proteins
- Cells
- Conditioned
- Culture Media
- Cultured
- Fibroblasts
- Gene Library
- Genetic
- Humans
- Interleukin-1
- Messenger
- Osteoarthritis
- Protein Biosynthesis
- RNA
- Synovial Membrane
- Transcription
- Transforming Growth Factor beta
- article
- bone morphogenetic protein
- bone morphogenetic protein 2
- controlled study
- cytokine
- dna library
- fibroblast
- gene expression regulation
- gene induction
- gingiva
- human
- human cell
- inflammatory disease
- interleukin 1
- messenger rna
- molecular cloning
- northern blotting
- periodontitis
- priority journal
- rheumatoid arthritis
- synovial fluid
- unclassified drug
Disciplines
- Genetics and Genomics