Mouse hepatitis virus type-2 infection in mice: An experimental model system of acute meningitis and hepatitis

Jayasri Das Sarma; Li Fu; Susan T. Hingley; Ehud Lavi

Mouse hepatitis virus type-2 infection in mice: An experimental model system of acute meningitis and hepatitis

Jayasri Das Sarma, Li Fu, Susan T. Hingley, Ehud Lavi

Department of Bio-Medical Sciences (PCOM)

Research output: Contribution to journal › Article › peer-review

Abstract

Infection with mouse hepatitis virus (MHV) strain A59 produces acute hepatitis, encephalitis, and chronic demyelination in mice. However, little is known about a closely related strain, MHV-2, which is only weakly neurotropic. To better understand the molecular basis of neurotropism of MHVs, we compared the pathogenesis and genomic sequence of MHV-2 with that of MHV-A59. Intracerebral injection of MHV-2 into 4-week-old C57B1/6 mice produces acute meningitis and hepatitis without encephalitis or chronic inflammatory demyelination. Sequence comparison between MHV-2 and MHV-A59 reveals 94-98% sequence identity of the replicase gene, 83-95% sequence identity of genes 2a, 3, 5b, 6, and 7, and marked difference in the sequence of genes, 2b, 4, and 5a. This information provides the basis for further studies exploring the mechanism of viral neurotropism and virus-induced demyelination. Â© 2001 Academic Press.

Original language	American English
Journal	Experimental and molecular pathology
Volume	71
State	Published - Jan 1 2001

Disciplines

Virology

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title = "Mouse hepatitis virus type-2 infection in mice: An experimental model system of acute meningitis and hepatitis",

abstract = " Infection with mouse hepatitis virus (MHV) strain A59 produces acute hepatitis, encephalitis, and chronic demyelination in mice. However, little is known about a closely related strain, MHV-2, which is only weakly neurotropic. To better understand the molecular basis of neurotropism of MHVs, we compared the pathogenesis and genomic sequence of MHV-2 with that of MHV-A59. Intracerebral injection of MHV-2 into 4-week-old C57B1/6 mice produces acute meningitis and hepatitis without encephalitis or chronic inflammatory demyelination. Sequence comparison between MHV-2 and MHV-A59 reveals 94-98\% sequence identity of the replicase gene, 83-95\% sequence identity of genes 2a, 3, 5b, 6, and 7, and marked difference in the sequence of genes, 2b, 4, and 5a. This information provides the basis for further studies exploring the mechanism of viral neurotropism and virus-induced demyelination. {\^A}{\textcopyright} 2001 Academic Press.",

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T1 - Mouse hepatitis virus type-2 infection in mice: An experimental model system of acute meningitis and hepatitis

AU - Das Sarma, Jayasri

AU - Fu, Li

AU - Hingley, Susan T.

AU - Lavi, Ehud

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Infection with mouse hepatitis virus (MHV) strain A59 produces acute hepatitis, encephalitis, and chronic demyelination in mice. However, little is known about a closely related strain, MHV-2, which is only weakly neurotropic. To better understand the molecular basis of neurotropism of MHVs, we compared the pathogenesis and genomic sequence of MHV-2 with that of MHV-A59. Intracerebral injection of MHV-2 into 4-week-old C57B1/6 mice produces acute meningitis and hepatitis without encephalitis or chronic inflammatory demyelination. Sequence comparison between MHV-2 and MHV-A59 reveals 94-98% sequence identity of the replicase gene, 83-95% sequence identity of genes 2a, 3, 5b, 6, and 7, and marked difference in the sequence of genes, 2b, 4, and 5a. This information provides the basis for further studies exploring the mechanism of viral neurotropism and virus-induced demyelination. Â© 2001 Academic Press.

AB - Infection with mouse hepatitis virus (MHV) strain A59 produces acute hepatitis, encephalitis, and chronic demyelination in mice. However, little is known about a closely related strain, MHV-2, which is only weakly neurotropic. To better understand the molecular basis of neurotropism of MHVs, we compared the pathogenesis and genomic sequence of MHV-2 with that of MHV-A59. Intracerebral injection of MHV-2 into 4-week-old C57B1/6 mice produces acute meningitis and hepatitis without encephalitis or chronic inflammatory demyelination. Sequence comparison between MHV-2 and MHV-A59 reveals 94-98% sequence identity of the replicase gene, 83-95% sequence identity of genes 2a, 3, 5b, 6, and 7, and marked difference in the sequence of genes, 2b, 4, and 5a. This information provides the basis for further studies exploring the mechanism of viral neurotropism and virus-induced demyelination. Â© 2001 Academic Press.

UR - https://digitalcommons.pcom.edu/scholarly_papers/428

M3 - Article

VL - 71

JO - Experimental and molecular pathology

JF - Experimental and molecular pathology

ER -

Mouse hepatitis virus type-2 infection in mice: An experimental model system of acute meningitis and hepatitis

Abstract

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