Myo/Nog cells are present in membranes from patients with proliferative vitreoretinopathy

Nathan Morrison; Lindsay Gugerty; Jacquelyn Gerhart; David Telander; Arturo Bravo Nuevo; Mindy George-Weinstein

doi:/article.aspx?articleid=2767862

Myo/Nog cells are present in membranes from patients with proliferative vitreoretinopathy

Nathan Morrison, Lindsay Gugerty, Jacquelyn Gerhart, David Telander, Arturo Bravo Nuevo, Mindy George-Weinstein

Research output: Contribution to conference › Presentation

Abstract

 Purpose : Proliferative vitreoretinopathy (PVR) is a complication of rhegmatogenous retinal detachment. The condition is characterized by the development of pre- or subretinal contractile membranes that may lead to redetachment of the retina. Membrane contraction has been attributed to skeletal muscle-like cells called myofibroblasts. Progenitors of contractile myofibrobasts in the lens are Myo/Nog cells named for their expression of mRNA for the skeletal muscle specific transcription factor MyoD and bone morphogenetic protein inhibitor noggin. The retina and choroid also contain Myo/Nog cells that respond to stress and injury. In this study we examined human PVR tissue for the presence of Myo/Nog cells and their expression of muscle proteins.
 
 Methods : PVR membranes were procured from patients in accordance with the Declaration of Helsinki and approved by the Institutional Review Board of the University of California, Davis. The tissue was embedded in paraffin, sectioned and fluorescently labeled with antibodies to the G8 antigen and noggin to identify Myo/Nog cells. Double labeling also was carried with G8 and antibodies to MyoD, alpha smooth muscle actin (α-SMA) and striated muscle myosin.
 
 Results : G8 and noggin were co-localized in a subpopulation of cells in sections from all three patients. The numbers of G8-positive (+)/noggin+ cells varied between patients. The percentages of G8+ cells that contained MyoD, α-SMA and myosin also differed in tissue samples. However, all cells containing muscle proteins were G8+. Some G8+/myosin+ cells appeared to be multinucleated.
 
 Conclusions : Myo/Nog cells are present among pigmented and non-pigmented cells in human PVR tissue. Variation in the numbers of Myo/Nog cells with MyoD, α-SMA and striated myosin suggests that the progenitor cells are in different stages of differentiation and maturation. These results lay the foundation for studies of the origin of Myo/Nog cells in PVR membranes and their contribution to retinal redetachment.
 
 This is a 2020 ARVO Annual Meeting abstract.

Original language	American English
DOIs	https://doi.org//article.aspx?articleid=2767862
State	Published - Jun 2020
Event	ARVO Annual Meeting 2020 - Honolulu, Hawaii Duration: Jun 1 2020 → …

Conference

Conference	ARVO Annual Meeting 2020
Period	6/1/20 → …

Disciplines

Medicine and Health Sciences
Ophthalmology

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Cite this

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title = "Myo/Nog cells are present in membranes from patients with proliferative vitreoretinopathy",

abstract = " Purpose : Proliferative vitreoretinopathy (PVR) is a complication of rhegmatogenous retinal detachment. The condition is characterized by the development of pre- or subretinal contractile membranes that may lead to redetachment of the retina. Membrane contraction has been attributed to skeletal muscle-like cells called myofibroblasts. Progenitors of contractile myofibrobasts in the lens are Myo/Nog cells named for their expression of mRNA for the skeletal muscle specific transcription factor MyoD and bone morphogenetic protein inhibitor noggin. The retina and choroid also contain Myo/Nog cells that respond to stress and injury. In this study we examined human PVR tissue for the presence of Myo/Nog cells and their expression of muscle proteins. Methods : PVR membranes were procured from patients in accordance with the Declaration of Helsinki and approved by the Institutional Review Board of the University of California, Davis. The tissue was embedded in paraffin, sectioned and fluorescently labeled with antibodies to the G8 antigen and noggin to identify Myo/Nog cells. Double labeling also was carried with G8 and antibodies to MyoD, alpha smooth muscle actin (α-SMA) and striated muscle myosin. Results : G8 and noggin were co-localized in a subpopulation of cells in sections from all three patients. The numbers of G8-positive (+)/noggin+ cells varied between patients. The percentages of G8+ cells that contained MyoD, α-SMA and myosin also differed in tissue samples. However, all cells containing muscle proteins were G8+. Some G8+/myosin+ cells appeared to be multinucleated. Conclusions : Myo/Nog cells are present among pigmented and non-pigmented cells in human PVR tissue. Variation in the numbers of Myo/Nog cells with MyoD, α-SMA and striated myosin suggests that the progenitor cells are in different stages of differentiation and maturation. These results lay the foundation for studies of the origin of Myo/Nog cells in PVR membranes and their contribution to retinal redetachment. This is a 2020 ARVO Annual Meeting abstract.",

author = "Nathan Morrison and Lindsay Gugerty and Jacquelyn Gerhart and David Telander and {Bravo Nuevo}, Arturo and Mindy George-Weinstein",

year = "2020",

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language = "American English",

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TY - CONF

T1 - Myo/Nog cells are present in membranes from patients with proliferative vitreoretinopathy

AU - Morrison, Nathan

AU - Gugerty, Lindsay

AU - Gerhart, Jacquelyn

AU - Telander, David

AU - Bravo Nuevo, Arturo

AU - George-Weinstein, Mindy

PY - 2020/6

Y1 - 2020/6

N2 - Purpose : Proliferative vitreoretinopathy (PVR) is a complication of rhegmatogenous retinal detachment. The condition is characterized by the development of pre- or subretinal contractile membranes that may lead to redetachment of the retina. Membrane contraction has been attributed to skeletal muscle-like cells called myofibroblasts. Progenitors of contractile myofibrobasts in the lens are Myo/Nog cells named for their expression of mRNA for the skeletal muscle specific transcription factor MyoD and bone morphogenetic protein inhibitor noggin. The retina and choroid also contain Myo/Nog cells that respond to stress and injury. In this study we examined human PVR tissue for the presence of Myo/Nog cells and their expression of muscle proteins. Methods : PVR membranes were procured from patients in accordance with the Declaration of Helsinki and approved by the Institutional Review Board of the University of California, Davis. The tissue was embedded in paraffin, sectioned and fluorescently labeled with antibodies to the G8 antigen and noggin to identify Myo/Nog cells. Double labeling also was carried with G8 and antibodies to MyoD, alpha smooth muscle actin (α-SMA) and striated muscle myosin. Results : G8 and noggin were co-localized in a subpopulation of cells in sections from all three patients. The numbers of G8-positive (+)/noggin+ cells varied between patients. The percentages of G8+ cells that contained MyoD, α-SMA and myosin also differed in tissue samples. However, all cells containing muscle proteins were G8+. Some G8+/myosin+ cells appeared to be multinucleated. Conclusions : Myo/Nog cells are present among pigmented and non-pigmented cells in human PVR tissue. Variation in the numbers of Myo/Nog cells with MyoD, α-SMA and striated myosin suggests that the progenitor cells are in different stages of differentiation and maturation. These results lay the foundation for studies of the origin of Myo/Nog cells in PVR membranes and their contribution to retinal redetachment. This is a 2020 ARVO Annual Meeting abstract.

AB - Purpose : Proliferative vitreoretinopathy (PVR) is a complication of rhegmatogenous retinal detachment. The condition is characterized by the development of pre- or subretinal contractile membranes that may lead to redetachment of the retina. Membrane contraction has been attributed to skeletal muscle-like cells called myofibroblasts. Progenitors of contractile myofibrobasts in the lens are Myo/Nog cells named for their expression of mRNA for the skeletal muscle specific transcription factor MyoD and bone morphogenetic protein inhibitor noggin. The retina and choroid also contain Myo/Nog cells that respond to stress and injury. In this study we examined human PVR tissue for the presence of Myo/Nog cells and their expression of muscle proteins. Methods : PVR membranes were procured from patients in accordance with the Declaration of Helsinki and approved by the Institutional Review Board of the University of California, Davis. The tissue was embedded in paraffin, sectioned and fluorescently labeled with antibodies to the G8 antigen and noggin to identify Myo/Nog cells. Double labeling also was carried with G8 and antibodies to MyoD, alpha smooth muscle actin (α-SMA) and striated muscle myosin. Results : G8 and noggin were co-localized in a subpopulation of cells in sections from all three patients. The numbers of G8-positive (+)/noggin+ cells varied between patients. The percentages of G8+ cells that contained MyoD, α-SMA and myosin also differed in tissue samples. However, all cells containing muscle proteins were G8+. Some G8+/myosin+ cells appeared to be multinucleated. Conclusions : Myo/Nog cells are present among pigmented and non-pigmented cells in human PVR tissue. Variation in the numbers of Myo/Nog cells with MyoD, α-SMA and striated myosin suggests that the progenitor cells are in different stages of differentiation and maturation. These results lay the foundation for studies of the origin of Myo/Nog cells in PVR membranes and their contribution to retinal redetachment. This is a 2020 ARVO Annual Meeting abstract.

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