Myo/Nog cells respond to photoreceptor cell death in the murine model of retinitis pigmentosa

Diana Crowley; Samantha Murad; Mary Woodruff; Sarah Coughlan; E-Jine Tsai; Rushil Brahmbhatt; Paul Lecker; Grzegorz Gorski; Jacquelyn Gerhart; Mindy George-Weinstein; Arturo Bravo Nuevo

doi:/article.aspx?articleid=2767432

Myo/Nog cells respond to photoreceptor cell death in the murine model of retinitis pigmentosa

Diana Crowley, Samantha Murad, Mary Woodruff, Sarah Coughlan, E-Jine Tsai, Rushil Brahmbhatt, Paul Lecker, Grzegorz Gorski, Jacquelyn Gerhart, Mindy George-Weinstein, Arturo Bravo Nuevo

Research output: Contribution to conference › Presentation

Abstract

 Purpose : Myo/Nog cells play an essential role in normal eye development. Previous studies have shown that they migrate to areas of stress and injury in the retina and are neuroprotective. In this study, we observed the behavior of Myo/Nog cells in a congenital murine model of retinitis pigmentosa (C3H mice), which results in the loss of photoreceptor cells within the outer nuclear layer of the retina (ONL).
 
 Methods : C3H and C57 control mice were assessed at weeks 2.5, 3, 4, 5, and 6 using Scotopic Electroretinographs (ERG), Ocular Computer Topography (OCT), and immunofluorescence microscopy. Enucleated eyes were fixed in paraformaldehyde and cryo-sectioned. Cryosections were labeled with Myo/Nog specific G8 monoclonal antibody.
 
 Results : ERGs demonstrated decreased amplitudes of the A and B waves in the C3H group compared to the C57 group (p<0.05). OCTs revealed a significant difference in retinal thickness between the two groups (p<0.05). Progressive thinning of the retina in the C3H mice was confirmed by microscopy. Myo/Nog cells were significantly more numerous in C3H mice compared to controls (p<0.05). The majority of Myo/Nog cells were found in the outer nuclear layer and within the neighboring choroid.
 
 Conclusions : Progressive retinal degeneration and visual deterioration in C3H mice is accompanied by an increase in Myo/Nog cells. They accumulate in parallel with an increase in photoreceptor cell death in the ONL. These findings are consistent with the behaviors of Myo/Nog cells in other forms of retinal stress and injury. Future studies will characterize the functions of Myo/Nog cells in this severe model of retinitis pigmentosa.
 
 This is a 2020 ARVO Annual Meeting abstract.

Original language	American English
DOIs	https://doi.org//article.aspx?articleid=2767432
State	Published - Jun 2020
Event	ARVO 2020 Annual Meeting - Baltimore, MD Duration: Jun 1 2020 → …

Conference

Conference	ARVO 2020 Annual Meeting
Period	6/1/20 → …

Disciplines

Medicine and Health Sciences
Ophthalmology

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/article.aspx?articleid=2767432License: Unspecified

Cite this

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title = "Myo/Nog cells respond to photoreceptor cell death in the murine model of retinitis pigmentosa",

abstract = " Purpose : Myo/Nog cells play an essential role in normal eye development. Previous studies have shown that they migrate to areas of stress and injury in the retina and are neuroprotective. In this study, we observed the behavior of Myo/Nog cells in a congenital murine model of retinitis pigmentosa (C3H mice), which results in the loss of photoreceptor cells within the outer nuclear layer of the retina (ONL). Methods : C3H and C57 control mice were assessed at weeks 2.5, 3, 4, 5, and 6 using Scotopic Electroretinographs (ERG), Ocular Computer Topography (OCT), and immunofluorescence microscopy. Enucleated eyes were fixed in paraformaldehyde and cryo-sectioned. Cryosections were labeled with Myo/Nog specific G8 monoclonal antibody. Results : ERGs demonstrated decreased amplitudes of the A and B waves in the C3H group compared to the C57 group (p<0.05). OCTs revealed a significant difference in retinal thickness between the two groups (p<0.05). Progressive thinning of the retina in the C3H mice was confirmed by microscopy. Myo/Nog cells were significantly more numerous in C3H mice compared to controls (p<0.05). The majority of Myo/Nog cells were found in the outer nuclear layer and within the neighboring choroid. Conclusions : Progressive retinal degeneration and visual deterioration in C3H mice is accompanied by an increase in Myo/Nog cells. They accumulate in parallel with an increase in photoreceptor cell death in the ONL. These findings are consistent with the behaviors of Myo/Nog cells in other forms of retinal stress and injury. Future studies will characterize the functions of Myo/Nog cells in this severe model of retinitis pigmentosa. This is a 2020 ARVO Annual Meeting abstract.",

author = "Diana Crowley and Samantha Murad and Mary Woodruff and Sarah Coughlan and E-Jine Tsai and Rushil Brahmbhatt and Paul Lecker and Grzegorz Gorski and Jacquelyn Gerhart and Mindy George-Weinstein and {Bravo Nuevo}, Arturo",

year = "2020",

month = jun,

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language = "American English",

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TY - CONF

T1 - Myo/Nog cells respond to photoreceptor cell death in the murine model of retinitis pigmentosa

AU - Crowley, Diana

AU - Murad, Samantha

AU - Woodruff, Mary

AU - Coughlan, Sarah

AU - Tsai, E-Jine

AU - Brahmbhatt, Rushil

AU - Lecker, Paul

AU - Gorski, Grzegorz

AU - Gerhart, Jacquelyn

AU - George-Weinstein, Mindy

AU - Bravo Nuevo, Arturo

PY - 2020/6

Y1 - 2020/6

N2 - Purpose : Myo/Nog cells play an essential role in normal eye development. Previous studies have shown that they migrate to areas of stress and injury in the retina and are neuroprotective. In this study, we observed the behavior of Myo/Nog cells in a congenital murine model of retinitis pigmentosa (C3H mice), which results in the loss of photoreceptor cells within the outer nuclear layer of the retina (ONL). Methods : C3H and C57 control mice were assessed at weeks 2.5, 3, 4, 5, and 6 using Scotopic Electroretinographs (ERG), Ocular Computer Topography (OCT), and immunofluorescence microscopy. Enucleated eyes were fixed in paraformaldehyde and cryo-sectioned. Cryosections were labeled with Myo/Nog specific G8 monoclonal antibody. Results : ERGs demonstrated decreased amplitudes of the A and B waves in the C3H group compared to the C57 group (p<0.05). OCTs revealed a significant difference in retinal thickness between the two groups (p<0.05). Progressive thinning of the retina in the C3H mice was confirmed by microscopy. Myo/Nog cells were significantly more numerous in C3H mice compared to controls (p<0.05). The majority of Myo/Nog cells were found in the outer nuclear layer and within the neighboring choroid. Conclusions : Progressive retinal degeneration and visual deterioration in C3H mice is accompanied by an increase in Myo/Nog cells. They accumulate in parallel with an increase in photoreceptor cell death in the ONL. These findings are consistent with the behaviors of Myo/Nog cells in other forms of retinal stress and injury. Future studies will characterize the functions of Myo/Nog cells in this severe model of retinitis pigmentosa. This is a 2020 ARVO Annual Meeting abstract.

AB - Purpose : Myo/Nog cells play an essential role in normal eye development. Previous studies have shown that they migrate to areas of stress and injury in the retina and are neuroprotective. In this study, we observed the behavior of Myo/Nog cells in a congenital murine model of retinitis pigmentosa (C3H mice), which results in the loss of photoreceptor cells within the outer nuclear layer of the retina (ONL). Methods : C3H and C57 control mice were assessed at weeks 2.5, 3, 4, 5, and 6 using Scotopic Electroretinographs (ERG), Ocular Computer Topography (OCT), and immunofluorescence microscopy. Enucleated eyes were fixed in paraformaldehyde and cryo-sectioned. Cryosections were labeled with Myo/Nog specific G8 monoclonal antibody. Results : ERGs demonstrated decreased amplitudes of the A and B waves in the C3H group compared to the C57 group (p<0.05). OCTs revealed a significant difference in retinal thickness between the two groups (p<0.05). Progressive thinning of the retina in the C3H mice was confirmed by microscopy. Myo/Nog cells were significantly more numerous in C3H mice compared to controls (p<0.05). The majority of Myo/Nog cells were found in the outer nuclear layer and within the neighboring choroid. Conclusions : Progressive retinal degeneration and visual deterioration in C3H mice is accompanied by an increase in Myo/Nog cells. They accumulate in parallel with an increase in photoreceptor cell death in the ONL. These findings are consistent with the behaviors of Myo/Nog cells in other forms of retinal stress and injury. Future studies will characterize the functions of Myo/Nog cells in this severe model of retinitis pigmentosa. This is a 2020 ARVO Annual Meeting abstract.

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M3 - Presentation

T2 - ARVO 2020 Annual Meeting

Y2 - 1 June 2020

ER -