NF-kB and ZBP-89 regulate MMP-3 expression via a polymorphic site in the promoter

Ruth C. Borghaei; G. Gorski; Masoud Javadi

NF-kB and ZBP-89 regulate MMP-3 expression via a polymorphic site in the promoter

Ruth C. Borghaei, G. Gorski, Masoud Javadi

Department of Bio-Medical Sciences (PCOM)

Research output: Contribution to journal › Article › peer-review

Abstract

A 5T/6T polymorphism in the human MMP-3 promoter affects gene expression and impacts the risk and/or severity of various pathological conditions. Chromatin immunoprecipitation (ChIP) in human fibroblasts homozygous for the 6T site demonstrate that it is bound by NF-kB and ZBP-89 transcription factors in its native chromatin. ChIP in COS-1 cells transfected with plasmids containing the 5T and 6T sites in the context of 2 kb of the MMP-3 promoter showed that NF-kB p50 binds preferentially to the 6T site, while more ZBP-89 binding is detected to the 5T site. Over-expressed ZBP-89 increased transcription from the 5T promoter but not from the 6T, while NF-kB decreased transcription from both promoters, even in the presence of excess ZBP-89. A model is suggested in which the physiological impact of the polymorphism is dependent on the relative levels and activities of these competing factors in various cell types and conditions.

Original language	American English
Journal	Biochemical and biophysical research communications
Volume	382
State	Published - Jan 1 2009

Keywords

Matrix metalloproteinase
NF-kB
Polymorphism
Stromelysin-1
Transcription factor
ZBP-89
ZNF148
immunoglobulin enhancer binding protein
stromelysin
unclassified drug
zinc binding protein
zinc binding protein 89
DNA binding protein
ZNF148 protein
human
animal cell
article
binding site
cell strain COS1
chromatin immunoprecipitation
controlled study
enzyme regulation
genetic polymorphism
genetic transcription
genetic transfection
human cell
nonhuman
plasmid
priority journal
promoter region
protein binding
protein expression
regulatory mechanism
animal
Cercopithecus
gene expression regulation
genetics
metabolism
Animals
Cercopithecus aethiops
COS Cells
DNA-Binding Proteins
Humans
Matrix Metalloproteinase 3
NF-kappa B
Genetic
Promoter Regions
Transcription Factors
Transcription

Disciplines

Genetics and Genomics

Cite this

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title = "NF-kB and ZBP-89 regulate MMP-3 expression via a polymorphic site in the promoter",

abstract = " A 5T/6T polymorphism in the human MMP-3 promoter affects gene expression and impacts the risk and/or severity of various pathological conditions. Chromatin immunoprecipitation (ChIP) in human fibroblasts homozygous for the 6T site demonstrate that it is bound by NF-kB and ZBP-89 transcription factors in its native chromatin. ChIP in COS-1 cells transfected with plasmids containing the 5T and 6T sites in the context of 2 kb of the MMP-3 promoter showed that NF-kB p50 binds preferentially to the 6T site, while more ZBP-89 binding is detected to the 5T site. Over-expressed ZBP-89 increased transcription from the 5T promoter but not from the 6T, while NF-kB decreased transcription from both promoters, even in the presence of excess ZBP-89. A model is suggested in which the physiological impact of the polymorphism is dependent on the relative levels and activities of these competing factors in various cell types and conditions.",

keywords = "Matrix metalloproteinase, NF-kB, Polymorphism, Stromelysin-1, Transcription factor, ZBP-89, ZNF148, immunoglobulin enhancer binding protein, stromelysin, unclassified drug, zinc binding protein, zinc binding protein 89, DNA binding protein, ZNF148 protein, human, animal cell, article, binding site, cell strain COS1, chromatin immunoprecipitation, controlled study, enzyme regulation, genetic polymorphism, genetic transcription, genetic transfection, human cell, nonhuman, plasmid, priority journal, promoter region, protein binding, protein expression, regulatory mechanism, animal, Cercopithecus, gene expression regulation, genetics, metabolism, Animals, Cercopithecus aethiops, COS Cells, DNA-Binding Proteins, Humans, Matrix Metalloproteinase 3, NF-kappa B, Genetic, Promoter Regions, Transcription Factors, Transcription",

author = "Borghaei, \{Ruth C.\} and G. Gorski and Masoud Javadi",

year = "2009",

month = jan,

day = "1",

language = "American English",

volume = "382",

journal = "Biochemical and biophysical research communications",

}

TY - JOUR

T1 - NF-kB and ZBP-89 regulate MMP-3 expression via a polymorphic site in the promoter

AU - Borghaei, Ruth C.

AU - Gorski, G.

AU - Javadi, Masoud

PY - 2009/1/1

Y1 - 2009/1/1

N2 - A 5T/6T polymorphism in the human MMP-3 promoter affects gene expression and impacts the risk and/or severity of various pathological conditions. Chromatin immunoprecipitation (ChIP) in human fibroblasts homozygous for the 6T site demonstrate that it is bound by NF-kB and ZBP-89 transcription factors in its native chromatin. ChIP in COS-1 cells transfected with plasmids containing the 5T and 6T sites in the context of 2 kb of the MMP-3 promoter showed that NF-kB p50 binds preferentially to the 6T site, while more ZBP-89 binding is detected to the 5T site. Over-expressed ZBP-89 increased transcription from the 5T promoter but not from the 6T, while NF-kB decreased transcription from both promoters, even in the presence of excess ZBP-89. A model is suggested in which the physiological impact of the polymorphism is dependent on the relative levels and activities of these competing factors in various cell types and conditions.

AB - A 5T/6T polymorphism in the human MMP-3 promoter affects gene expression and impacts the risk and/or severity of various pathological conditions. Chromatin immunoprecipitation (ChIP) in human fibroblasts homozygous for the 6T site demonstrate that it is bound by NF-kB and ZBP-89 transcription factors in its native chromatin. ChIP in COS-1 cells transfected with plasmids containing the 5T and 6T sites in the context of 2 kb of the MMP-3 promoter showed that NF-kB p50 binds preferentially to the 6T site, while more ZBP-89 binding is detected to the 5T site. Over-expressed ZBP-89 increased transcription from the 5T promoter but not from the 6T, while NF-kB decreased transcription from both promoters, even in the presence of excess ZBP-89. A model is suggested in which the physiological impact of the polymorphism is dependent on the relative levels and activities of these competing factors in various cell types and conditions.

KW - Matrix metalloproteinase

KW - NF-kB

KW - Polymorphism

KW - Stromelysin-1

KW - Transcription factor

KW - ZBP-89

KW - ZNF148

KW - immunoglobulin enhancer binding protein

KW - stromelysin

KW - unclassified drug

KW - zinc binding protein

KW - zinc binding protein 89

KW - DNA binding protein

KW - ZNF148 protein

KW - human

KW - animal cell

KW - article

KW - binding site

KW - cell strain COS1

KW - chromatin immunoprecipitation

KW - controlled study

KW - enzyme regulation

KW - genetic polymorphism

KW - genetic transcription

KW - genetic transfection

KW - human cell

KW - nonhuman

KW - plasmid

KW - priority journal

KW - promoter region

KW - protein binding

KW - protein expression

KW - regulatory mechanism

KW - animal

KW - Cercopithecus

KW - gene expression regulation

KW - genetics

KW - metabolism

KW - Animals

KW - Cercopithecus aethiops

KW - COS Cells

KW - DNA-Binding Proteins

KW - Humans

KW - Matrix Metalloproteinase 3

KW - NF-kappa B

KW - Genetic

KW - Promoter Regions

KW - Transcription Factors

KW - Transcription

UR - https://digitalcommons.pcom.edu/scholarly_papers/1221

M3 - Article

VL - 382

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

ER -

NF-kB and ZBP-89 regulate MMP-3 expression via a polymorphic site in the promoter

Abstract

Keywords

Disciplines

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