Organization and chromosomal localization of a B1-like containing repeat of Microtus subarvalis

V. I. Mayorov; L. R. Adkison; N. V. Vorobyeva; Eu A. Khrapov; N. G. Kholodhov; I. B. Rogozin; T. B. Nesterova; A. I. Protopopov; O. V. Sablina; A. S. Graphodatsky; S. M. Zakian

doi:10.1007/s003359900176

Organization and chromosomal localization of a B1-like containing repeat of Microtus subarvalis

V. I. Mayorov
, L. R. Adkison
, N. V. Vorobyeva
, Eu A. Khrapov
, N. G. Kholodhov
, I. B. Rogozin
, T. B. Nesterova
, A. I. Protopopov
, O. V. Sablina
, A. S. Graphodatsky
, S. M. Zakian

Department of Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

Abstract

A repetitive DNA sequence, MS2, was isolated from EcoRI-digested genomic DNA of the vole, Microtus subarvalis. The fragment was cloned and sequenced. Sequence analysis of this 1194-bp fragment revealed a 156-bp region demonstrating a 55% homology with the mouse B1 repeat. The remaining MS2 sequence shows no significant homology with other known Gen-Bank sequences. The results of in situ hybridization of MS2 on vole metaphase chromosomes indicate the fragment is confined to heterochromatin blocks of the sex chromosomes in all but one species (M. arvalis). Distribution of MS2 sequences provides evidence for heterogeneity of the giant heterochromatin blocks of the XY Chromosomes (Chrs) in voles, for the unique cluster-like localization of MS2 within these blocks.

Original language	English
Pages (from-to)	593-597
Number of pages	5
Journal	Mammalian Genome
Volume	7
Issue number	8
DOIs	https://doi.org/10.1007/s003359900176
State	Published - 1996

ASJC Scopus Subject Areas

Genetics

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@article{64461a39081e496ba16749595f6a3213,

title = "Organization and chromosomal localization of a B1-like containing repeat of Microtus subarvalis",

abstract = "A repetitive DNA sequence, MS2, was isolated from EcoRI-digested genomic DNA of the vole, Microtus subarvalis. The fragment was cloned and sequenced. Sequence analysis of this 1194-bp fragment revealed a 156-bp region demonstrating a 55\% homology with the mouse B1 repeat. The remaining MS2 sequence shows no significant homology with other known Gen-Bank sequences. The results of in situ hybridization of MS2 on vole metaphase chromosomes indicate the fragment is confined to heterochromatin blocks of the sex chromosomes in all but one species (M. arvalis). Distribution of MS2 sequences provides evidence for heterogeneity of the giant heterochromatin blocks of the XY Chromosomes (Chrs) in voles, for the unique cluster-like localization of MS2 within these blocks.",

author = "Mayorov, \{V. I.\} and Adkison, \{L. R.\} and Vorobyeva, \{N. V.\} and Khrapov, \{Eu A.\} and Kholodhov, \{N. G.\} and Rogozin, \{I. B.\} and Nesterova, \{T. B.\} and Protopopov, \{A. I.\} and Sablina, \{O. V.\} and Graphodatsky, \{A. S.\} and Zakian, \{S. M.\}",

year = "1996",

doi = "10.1007/s003359900176",

language = "English",

volume = "7",

pages = "593--597",

journal = "Mammalian Genome",

issn = "0938-8990",

number = "8",

}

TY - JOUR

T1 - Organization and chromosomal localization of a B1-like containing repeat of Microtus subarvalis

AU - Mayorov, V. I.

AU - Adkison, L. R.

AU - Vorobyeva, N. V.

AU - Khrapov, Eu A.

AU - Kholodhov, N. G.

AU - Rogozin, I. B.

AU - Nesterova, T. B.

AU - Protopopov, A. I.

AU - Sablina, O. V.

AU - Graphodatsky, A. S.

AU - Zakian, S. M.

PY - 1996

Y1 - 1996

N2 - A repetitive DNA sequence, MS2, was isolated from EcoRI-digested genomic DNA of the vole, Microtus subarvalis. The fragment was cloned and sequenced. Sequence analysis of this 1194-bp fragment revealed a 156-bp region demonstrating a 55% homology with the mouse B1 repeat. The remaining MS2 sequence shows no significant homology with other known Gen-Bank sequences. The results of in situ hybridization of MS2 on vole metaphase chromosomes indicate the fragment is confined to heterochromatin blocks of the sex chromosomes in all but one species (M. arvalis). Distribution of MS2 sequences provides evidence for heterogeneity of the giant heterochromatin blocks of the XY Chromosomes (Chrs) in voles, for the unique cluster-like localization of MS2 within these blocks.

AB - A repetitive DNA sequence, MS2, was isolated from EcoRI-digested genomic DNA of the vole, Microtus subarvalis. The fragment was cloned and sequenced. Sequence analysis of this 1194-bp fragment revealed a 156-bp region demonstrating a 55% homology with the mouse B1 repeat. The remaining MS2 sequence shows no significant homology with other known Gen-Bank sequences. The results of in situ hybridization of MS2 on vole metaphase chromosomes indicate the fragment is confined to heterochromatin blocks of the sex chromosomes in all but one species (M. arvalis). Distribution of MS2 sequences provides evidence for heterogeneity of the giant heterochromatin blocks of the XY Chromosomes (Chrs) in voles, for the unique cluster-like localization of MS2 within these blocks.

UR - https://www.scopus.com/pages/publications/0030215495

UR - https://www.scopus.com/pages/publications/0030215495#tab=citedBy

U2 - 10.1007/s003359900176

DO - 10.1007/s003359900176

M3 - Article

C2 - 8678980

AN - SCOPUS:0030215495

SN - 0938-8990

VL - 7

SP - 593

EP - 597

JO - Mammalian Genome

JF - Mammalian Genome

IS - 8

ER -

Organization and chromosomal localization of a B1-like containing repeat of Microtus subarvalis

Abstract

ASJC Scopus Subject Areas

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