TY - JOUR
T1 - Preparative fractionation of t and b lymphocytes of the syrian Golden hamster with soybean agglutinin
AU - Weppner, W. A.
AU - Adkison, L. R.
PY - 1980
Y1 - 1980
N2 - T and B lymphocytes of the Syrian golden hamster were separated from spleen cell preparations on the basis of their differential agglutinability with the lectin soybean agglutinin. Only B lymphocytes were agglutinated by this lectin, and they could be separated from the unagglutinated T lymphocytes by sedimentation through 50% heat inactivated calf serum at unit gravity. The B lymphocyte aggregates could be dissociated into single cells that were viable and functional after treatment with 0.5 M galactose. The isolated cell fractions were characterized by their blastogenic response to various T cell and B cell specific mitogens and by the presence or absence of cell surface IgG. © 1980 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
AB - T and B lymphocytes of the Syrian golden hamster were separated from spleen cell preparations on the basis of their differential agglutinability with the lectin soybean agglutinin. Only B lymphocytes were agglutinated by this lectin, and they could be separated from the unagglutinated T lymphocytes by sedimentation through 50% heat inactivated calf serum at unit gravity. The B lymphocyte aggregates could be dissociated into single cells that were viable and functional after treatment with 0.5 M galactose. The isolated cell fractions were characterized by their blastogenic response to various T cell and B cell specific mitogens and by the presence or absence of cell surface IgG. © 1980 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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U2 - 10.3109/08820138009109685
DO - 10.3109/08820138009109685
M3 - Article
C2 - 6972346
AN - SCOPUS:84907109238
SN - 0882-0139
VL - 9
SP - 759
EP - 768
JO - Immunological Investigations
JF - Immunological Investigations
IS - 8
ER -