Abstract
The quantitative determination of caffeic acid phenethyl ester (CAPE) and its fluorinated derivative (FCAPE) from rat plasma using ultra-performance liquid chromatography with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) is reported. CAPE and FCAPE were extracted using ethyl acetate in the presence of methyl caffeate (MC) as internal standard. Separation was achieved using a C(18) column (2.1 mm x 50 mm, 1.7 microm) and gradient elution with water and acetonitrile containing 0.2% and 0.1% formic acid, respectively. A non-linear response over a broad concentration range (1-1000 ng/ml, r(2)>0.995 using a quadratic regression model and 1/concentration weighting) was obtained. The inter-day and intra-day variability for CAPE and FCAPE were found to be less than 14.2% and 9.5%, respectively. Data are presented to illustrate the practicality of the method for the pharmacokinetic evaluation of CAPE and FCAPE after intravenous administration to rats.
Original language | American English |
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Journal | Journal of chromatography.B, Analytical technologies in the biomedical and life sciences |
Volume | 867 |
State | Published - Jan 1 2008 |
Keywords
- Animals
- Caffeic Acids/blood/chemistry
- Chromatography
- Liquid/methods
- Fluorine/chemistry
- Male
- Phenylethyl Alcohol/analogs & derivatives/blood/chemistry
- Rats
- Sprague-Dawley
- Reproducibility of Results
- Spectrometry
- Mass
- Electrospray Ionization/methods
- Tandem Mass Spectrometry/methods
Disciplines
- Pharmacy and Pharmaceutical Sciences