Robust, High-Throughput Solution Structural Analyses by Small Angle X-Ray Scattering (SAXS)

Greg L. Hura; Angeli L. Menon; Michal Hammel; Robert P. Rambo; Farris L. Poole; Susan E. Tsutakawa; Francis E. Jenney; Scott Classen; Kenneth A. Frankel; Robert C. Hopkins; Sung-Jae Yang; Joseph W. Scott; Bret D. Dillard; Michael W. W. Adams; John A. Tainer

Robust, High-Throughput Solution Structural Analyses by Small Angle X-Ray Scattering (SAXS)

Greg L. Hura, Angeli L. Menon, Michal Hammel, Robert P. Rambo, Farris L. Poole, Susan E. Tsutakawa, Francis E. Jenney, Scott Classen, Kenneth A. Frankel, Robert C. Hopkins, Sung-Jae Yang, Joseph W. Scott, Bret D. Dillard, Michael W. W. Adams, John A. Tainer

Department of Bio-Medical Sciences (PCOM)

Research output: Contribution to journal › Article › peer-review

Abstract

We present an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering (SAXS). Our SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data collection and data analysis, and couples structural analysis with automated archiving. We subjected 50 representative proteins, mostly from Pyrococcus furiosus, to this pipeline and found that 30 were multimeric structures in solution. SAXS analysis allowed us to distinguish aggregated and unfolded proteins, define global structural parameters and oligomeric states for most samples, identify shapes and similar structures for 25 unknown structures, and determine envelopes for 41 proteins. We believe that high-throughput SAXS is an enabling technology that may change the way that structural genomics research is done.

Original language	American English
Journal	Nature Methods
Volume	6
State	Published - Aug 1 2009

Keywords

Bacterial Proteins
Equipment Design
Models
Molecular
Protein Conformation
Proteins
Pyrococcus furiosus
Scattering
Small Angle
X-Ray Diffraction

Disciplines

Genetics and Genomics
Medicine and Health Sciences

Cite this

@article{3b3966df029f4f88b37447b812e98605,

title = "Robust, High-Throughput Solution Structural Analyses by Small Angle X-Ray Scattering (SAXS)",

abstract = " We present an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering (SAXS). Our SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data collection and data analysis, and couples structural analysis with automated archiving. We subjected 50 representative proteins, mostly from Pyrococcus furiosus, to this pipeline and found that 30 were multimeric structures in solution. SAXS analysis allowed us to distinguish aggregated and unfolded proteins, define global structural parameters and oligomeric states for most samples, identify shapes and similar structures for 25 unknown structures, and determine envelopes for 41 proteins. We believe that high-throughput SAXS is an enabling technology that may change the way that structural genomics research is done.",

keywords = "Bacterial Proteins, Equipment Design, Models, Molecular, Protein Conformation, Proteins, Pyrococcus furiosus, Scattering, Small Angle, X-Ray Diffraction",

author = "Hura, {Greg L.} and Menon, {Angeli L.} and Michal Hammel and Rambo, {Robert P.} and Poole, {Farris L.} and Tsutakawa, {Susan E.} and Jenney, {Francis E.} and Scott Classen and Frankel, {Kenneth A.} and Hopkins, {Robert C.} and Sung-Jae Yang and Scott, {Joseph W.} and Dillard, {Bret D.} and Adams, {Michael W. W.} and Tainer, {John A.}",

year = "2009",

month = aug,

day = "1",

language = "American English",

volume = "6",

journal = "Nature Methods",

}

TY - JOUR

T1 - Robust, High-Throughput Solution Structural Analyses by Small Angle X-Ray Scattering (SAXS)

AU - Hura, Greg L.

AU - Menon, Angeli L.

AU - Hammel, Michal

AU - Rambo, Robert P.

AU - Poole, Farris L.

AU - Tsutakawa, Susan E.

AU - Jenney, Francis E.

AU - Classen, Scott

AU - Frankel, Kenneth A.

AU - Hopkins, Robert C.

AU - Yang, Sung-Jae

AU - Scott, Joseph W.

AU - Dillard, Bret D.

AU - Adams, Michael W. W.

AU - Tainer, John A.

PY - 2009/8/1

Y1 - 2009/8/1

N2 - We present an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering (SAXS). Our SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data collection and data analysis, and couples structural analysis with automated archiving. We subjected 50 representative proteins, mostly from Pyrococcus furiosus, to this pipeline and found that 30 were multimeric structures in solution. SAXS analysis allowed us to distinguish aggregated and unfolded proteins, define global structural parameters and oligomeric states for most samples, identify shapes and similar structures for 25 unknown structures, and determine envelopes for 41 proteins. We believe that high-throughput SAXS is an enabling technology that may change the way that structural genomics research is done.

AB - We present an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering (SAXS). Our SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data collection and data analysis, and couples structural analysis with automated archiving. We subjected 50 representative proteins, mostly from Pyrococcus furiosus, to this pipeline and found that 30 were multimeric structures in solution. SAXS analysis allowed us to distinguish aggregated and unfolded proteins, define global structural parameters and oligomeric states for most samples, identify shapes and similar structures for 25 unknown structures, and determine envelopes for 41 proteins. We believe that high-throughput SAXS is an enabling technology that may change the way that structural genomics research is done.

KW - Bacterial Proteins

KW - Equipment Design

KW - Models

KW - Molecular

KW - Protein Conformation

KW - Proteins

KW - Pyrococcus furiosus

KW - Scattering

KW - Small Angle

KW - X-Ray Diffraction

UR - https://digitalcommons.pcom.edu/scholarly_papers/57

M3 - Article

VL - 6

JO - Nature Methods

JF - Nature Methods

ER -

Robust, High-Throughput Solution Structural Analyses by Small Angle X-Ray Scattering (SAXS)

Abstract

Keywords

Disciplines

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