Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution

Laura-Lee Clancy Kelley; Bret D. Dillard; Wolfram Tempel; Lirong Chen; Neil Shaw; Doowon Lee; M. Gary Newton; Frank J. Sugar; Francis E. Jenney; Han Seung Lee; Claudia Shah; Farris L. Poole; Michael W. W. Adams; Jane S. Richardson; David C. Richardson; Zhi-Jie Liu; Bi-Cheng Wang; John Rose

Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution

Laura-Lee Clancy Kelley, Bret D. Dillard, Wolfram Tempel, Lirong Chen, Neil Shaw, Doowon Lee, M. Gary Newton, Frank J. Sugar, Francis E. Jenney, Han Seung Lee, Claudia Shah, Farris L. Poole, Michael W. W. Adams, Jane S. Richardson, David C. Richardson, Zhi-Jie Liu, Bi-Cheng Wang, John Rose

Department of Bio-Medical Sciences (PCOM)

Research output: Contribution to journal › Article › peer-review

Abstract

The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.

Original language	American English
Journal	Acta Crystallographica Section F, Structural Biology and Crystallization Communications
Volume	63
State	Published - Jul 1 2007

Keywords

Archaeal Proteins
Cloning
Molecular
Crystallization
Crystallography
X-Ray
Open Reading Frames
Protein Structure
Secondary
Pyrococcus furiosus

Disciplines

Genomics
Medicine and Health Sciences
Structural Biology

Cite this

Kelley, L.-L. C., Dillard, B. D., Tempel, W., Chen, L., Shaw, N., Lee, D., Newton, M. G., Sugar, F. J., Jenney, F. E., Lee, H. S., Shah, C., Poole, F. L., Adams, M. W. W., Richardson, J. S., Richardson, D. C., Liu, Z.-J., Wang, B.-C., & Rose, J. (2007). Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution. Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 63.

Kelley, L-LC, Dillard, BD, Tempel, W, Chen, L, Shaw, N, Lee, D, Newton, MG, Sugar, FJ, Jenney, FE, Lee, HS, Shah, C, Poole, FL, Adams, MWW, Richardson, JS, Richardson, DC, Liu, Z-J, Wang, B-C & Rose, J 2007, 'Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution', Acta Crystallographica Section F, Structural Biology and Crystallization Communications, vol. 63.

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title = "Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution",

abstract = " The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.",

keywords = "Archaeal Proteins, Cloning, Molecular, Crystallization, Crystallography, X-Ray, Open Reading Frames, Protein Structure, Secondary, Pyrococcus furiosus",

author = "Kelley, {Laura-Lee Clancy} and Dillard, {Bret D.} and Wolfram Tempel and Lirong Chen and Neil Shaw and Doowon Lee and Newton, {M. Gary} and Sugar, {Frank J.} and Jenney, {Francis E.} and Lee, {Han Seung} and Claudia Shah and Poole, {Farris L.} and Adams, {Michael W. W.} and Richardson, {Jane S.} and Richardson, {David C.} and Zhi-Jie Liu and Bi-Cheng Wang and John Rose",

year = "2007",

month = jul,

day = "1",

language = "American English",

volume = "63",

journal = "Acta Crystallographica Section F, Structural Biology and Crystallization Communications",

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TY - JOUR

T1 - Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution

AU - Kelley, Laura-Lee Clancy

AU - Dillard, Bret D.

AU - Tempel, Wolfram

AU - Chen, Lirong

AU - Shaw, Neil

AU - Lee, Doowon

AU - Newton, M. Gary

AU - Sugar, Frank J.

AU - Jenney, Francis E.

AU - Lee, Han Seung

AU - Shah, Claudia

AU - Poole, Farris L.

AU - Adams, Michael W. W.

AU - Richardson, Jane S.

AU - Richardson, David C.

AU - Liu, Zhi-Jie

AU - Wang, Bi-Cheng

AU - Rose, John

PY - 2007/7/1

Y1 - 2007/7/1

N2 - The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.

AB - The hypothetical protein PF0899 is a 95-residue peptide from the hyperthermophilic archaeon Pyrococcus furiosus that represents a gene family with six members. P. furiosus ORF PF0899 has been cloned, expressed and crystallized and its structure has been determined by the Southeast Collaboratory for Structural Genomics (http://www.secsg.org). The structure was solved using the SCA2Structure pipeline from multiple data sets and has been refined to 1.85 A against the highest resolution data set collected (a presumed gold derivative), with a crystallographic R factor of 21.0% and R(free) of 24.0%. The refined structure shows some structural similarity to a wedge-shaped domain observed in the structure of the major capsid protein from bacteriophage HK97, suggesting that PF0899 may be a structural protein.

KW - Archaeal Proteins

KW - Cloning

KW - Molecular

KW - Crystallization

KW - Crystallography

KW - X-Ray

KW - Open Reading Frames

KW - Protein Structure

KW - Secondary

KW - Pyrococcus furiosus

UR - https://digitalcommons.pcom.edu/scholarly_papers/200

M3 - Article

VL - 63

JO - Acta Crystallographica Section F, Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F, Structural Biology and Crystallization Communications

ER -

Structure of the Hypothetical Protein PF0899 from Pyrococcus furiosus at 1.85 A Resolution

Abstract

Keywords

Disciplines

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