The Effects of Modulating eNOS Activity and Coupling on Leukocyte-endothelial Interactions in Rat Mesenteric Postcapillary Venules

Amber N. Koon; Maria Kern; Lindon H. Young; Brian Rueter; Edward S. Iames; Qian Chen

The Effects of Modulating eNOS Activity and Coupling on Leukocyte-endothelial Interactions in Rat Mesenteric Postcapillary Venules

Amber N. Koon, Maria Kern, Lindon H. Young, Brian Rueter, Edward S. Iames, Qian Chen

Department of Bio-Medical Sciences (PCOM)

Research output: Other contribution

Abstract

Background : Leukocyte-endothelial interactions associated with vascular injury are attenuated by endothelial-derived nitric oxide (NO). Endothelial NO synthase (eNOS) in the presence of tetrahydrobiopterin (BH4) produces NO from L-arginine and is termed eNOS coupling. However, when the ratio of dihydrobiopterin (BH2) to BH4 is increased, eNOS becomes uncoupled and produces superoxide instead of NO. Protein kinase C epsilon (PKC ε) positively regulates eNOS activity. This study examined modulating eNOS activity and coupling by superfusing BH2 (100 μM) by itself, combined with PKC ε activator (10μM) or PKC ε inhibitor, or combined with BH4 (100μM) and PKC ε activator in rat mesenteric venules.

Original language	American English
State	Published - May 2 2012

Keywords

Nitric Oxide Synthase
Endothelium
Leukocytes
Rats

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title = "The Effects of Modulating eNOS Activity and Coupling on Leukocyte-endothelial Interactions in Rat Mesenteric Postcapillary Venules",

abstract = " Background : Leukocyte-endothelial interactions associated with vascular injury are attenuated by endothelial-derived nitric oxide (NO). Endothelial NO synthase (eNOS) in the presence of tetrahydrobiopterin (BH4) produces NO from L-arginine and is termed eNOS coupling. However, when the ratio of dihydrobiopterin (BH2) to BH4 is increased, eNOS becomes uncoupled and produces superoxide instead of NO. Protein kinase C epsilon (PKC ε) positively regulates eNOS activity. This study examined modulating eNOS activity and coupling by superfusing BH2 (100 μM) by itself, combined with PKC ε activator (10μM) or PKC ε inhibitor, or combined with BH4 (100μM) and PKC ε activator in rat mesenteric venules.",

keywords = "Nitric Oxide Synthase, Endothelium, Leukocytes, Rats",

author = "Koon, {Amber N.} and Maria Kern and Young, {Lindon H.} and Brian Rueter and Iames, {Edward S.} and Qian Chen",

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T1 - The Effects of Modulating eNOS Activity and Coupling on Leukocyte-endothelial Interactions in Rat Mesenteric Postcapillary Venules

AU - Koon, Amber N.

AU - Kern, Maria

AU - Young, Lindon H.

AU - Rueter, Brian

AU - Iames, Edward S.

AU - Chen, Qian

PY - 2012/5/2

Y1 - 2012/5/2

N2 - Background : Leukocyte-endothelial interactions associated with vascular injury are attenuated by endothelial-derived nitric oxide (NO). Endothelial NO synthase (eNOS) in the presence of tetrahydrobiopterin (BH4) produces NO from L-arginine and is termed eNOS coupling. However, when the ratio of dihydrobiopterin (BH2) to BH4 is increased, eNOS becomes uncoupled and produces superoxide instead of NO. Protein kinase C epsilon (PKC ε) positively regulates eNOS activity. This study examined modulating eNOS activity and coupling by superfusing BH2 (100 μM) by itself, combined with PKC ε activator (10μM) or PKC ε inhibitor, or combined with BH4 (100μM) and PKC ε activator in rat mesenteric venules.

AB - Background : Leukocyte-endothelial interactions associated with vascular injury are attenuated by endothelial-derived nitric oxide (NO). Endothelial NO synthase (eNOS) in the presence of tetrahydrobiopterin (BH4) produces NO from L-arginine and is termed eNOS coupling. However, when the ratio of dihydrobiopterin (BH2) to BH4 is increased, eNOS becomes uncoupled and produces superoxide instead of NO. Protein kinase C epsilon (PKC ε) positively regulates eNOS activity. This study examined modulating eNOS activity and coupling by superfusing BH2 (100 μM) by itself, combined with PKC ε activator (10μM) or PKC ε inhibitor, or combined with BH4 (100μM) and PKC ε activator in rat mesenteric venules.

KW - Nitric Oxide Synthase

KW - Endothelium

KW - Leukocytes

KW - Rats

UR - https://digitalcommons.pcom.edu/research_day/research_day2012/research/12

M3 - Other contribution

ER -

The Effects of Modulating eNOS Activity and Coupling on Leukocyte-endothelial Interactions in Rat Mesenteric Postcapillary Venules

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Keywords

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