The Role of Endothelial Nitric Oxide Synthase (eNOS) Uncoupling on Leukocyte-Endothelial Interactions in Rat Mesenteric Postcapillary Venules

Maria Kern; Lindon H. Young; Brian Rueter; Edward S. Iames; Qian Chen

The Role of Endothelial Nitric Oxide Synthase (eNOS) Uncoupling on Leukocyte-Endothelial Interactions in Rat Mesenteric Postcapillary Venules

Maria Kern, Lindon H. Young, Brian Rueter, Edward S. Iames, Qian Chen

Department of Bio-Medical Sciences (PCOM)

Research output: Other contribution

Abstract

BACKGROUND: Endothelial derived nitric oxide (NO) is essential in the regulation of blood pressure and attenuates leukocyte-endothelial interactions associated with vascular injury. Endothelial NO synthase (eNOS) is coupled to L-arginine in the presence of tetrahydrobiopetrin (BH4) to produce NO. However, when BH4 is oxidized to dihydrobiopetrin (BH2) under conditions of oxidative stress, the ratio of BH2 to BH4 is increased causing the uncoupling of eNOS to use molecular oxygen as a substrate, instead of L-arginine, to produce superoxide.

Original language	American English
State	Published - Apr 27 2011

Keywords

Nitric Oxide Synthase
Endothelium
Leukocytes
Rats

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The Role of Endothelial Nitric Oxide Synthase (eNOS) Uncoupling oFinal published version, 14.4 KBLicense: CC BY

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title = "The Role of Endothelial Nitric Oxide Synthase (eNOS) Uncoupling on Leukocyte-Endothelial Interactions in Rat Mesenteric Postcapillary Venules",

abstract = " BACKGROUND: Endothelial derived nitric oxide (NO) is essential in the regulation of blood pressure and attenuates leukocyte-endothelial interactions associated with vascular injury. Endothelial NO synthase (eNOS) is coupled to L-arginine in the presence of tetrahydrobiopetrin (BH4) to produce NO. However, when BH4 is oxidized to dihydrobiopetrin (BH2) under conditions of oxidative stress, the ratio of BH2 to BH4 is increased causing the uncoupling of eNOS to use molecular oxygen as a substrate, instead of L-arginine, to produce superoxide.",

keywords = "Nitric Oxide Synthase, Endothelium, Leukocytes, Rats",

author = "Maria Kern and Young, {Lindon H.} and Brian Rueter and Iames, {Edward S.} and Qian Chen",

year = "2011",

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day = "27",

language = "American English",

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T1 - The Role of Endothelial Nitric Oxide Synthase (eNOS) Uncoupling on Leukocyte-Endothelial Interactions in Rat Mesenteric Postcapillary Venules

AU - Kern, Maria

AU - Young, Lindon H.

AU - Rueter, Brian

AU - Iames, Edward S.

AU - Chen, Qian

PY - 2011/4/27

Y1 - 2011/4/27

N2 - BACKGROUND: Endothelial derived nitric oxide (NO) is essential in the regulation of blood pressure and attenuates leukocyte-endothelial interactions associated with vascular injury. Endothelial NO synthase (eNOS) is coupled to L-arginine in the presence of tetrahydrobiopetrin (BH4) to produce NO. However, when BH4 is oxidized to dihydrobiopetrin (BH2) under conditions of oxidative stress, the ratio of BH2 to BH4 is increased causing the uncoupling of eNOS to use molecular oxygen as a substrate, instead of L-arginine, to produce superoxide.

AB - BACKGROUND: Endothelial derived nitric oxide (NO) is essential in the regulation of blood pressure and attenuates leukocyte-endothelial interactions associated with vascular injury. Endothelial NO synthase (eNOS) is coupled to L-arginine in the presence of tetrahydrobiopetrin (BH4) to produce NO. However, when BH4 is oxidized to dihydrobiopetrin (BH2) under conditions of oxidative stress, the ratio of BH2 to BH4 is increased causing the uncoupling of eNOS to use molecular oxygen as a substrate, instead of L-arginine, to produce superoxide.

KW - Nitric Oxide Synthase

KW - Endothelium

KW - Leukocytes

KW - Rats

UR - https://digitalcommons.pcom.edu/research_day/research_day/research/13

M3 - Other contribution

ER -

The Role of Endothelial Nitric Oxide Synthase (eNOS) Uncoupling on Leukocyte-Endothelial Interactions in Rat Mesenteric Postcapillary Venules

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Keywords

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