Abstract
The aim of this review is to summarize ways in which in vitro approaches have allowed us to investigate several aspects of gametogenesis in the male. In our laboratory, we have established both organ culture and cell co- culture methodologies and applied them to questions focused on cellular and molecular events important for development of primitive spermatogonia, or gonocytes, in testes of neonatal rats. We have described their postnatal reinitiation of mitosis and their migration to the basal lamina in anticipation of basal compartment formation and, through use of these in vitro systems, we have identified several mechanisms regulating these processes. These include matrix influence on mitosis and migration, adhesive mechanisms active between gonocytes and Sertoli cells, and involvement of the Kit receptor on germ cells and its ligand from Sertoli cells in supporting gonocyte migration, as described below.
Original language | American English |
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Journal | Theriogenology |
Volume | 49 |
State | Published - Jan 1 1998 |
Keywords
- Animalia
- Animals
- Coculture Techniques
- Epithelial Cells
- In vitro
- Male germ cells
- Migration
- Newborn
- Organ Culture Techniques
- Proliferation
- Rats
- Seminiferous Tubules
- Sertoli Cells
- Spermatogonia
- Spermatozoa
- Testis
- animal
- animal cell
- animal tissue
- cell adhesion
- cell maturation
- cell migration
- cell proliferation
- coculture
- conference paper
- controlled study
- cytology
- epithelium cell
- gametogenesis
- germ cell
- in vitro study
- male
- methodology
- mitosis
- newborn
- nonhuman
- organ culture
- physiology
- rat
- seminiferous tubule
- sertoli cell
- spermatogonium
- spermatozoon
- stem cell factor
- testis development
Disciplines
- Endocrinology